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. 2006 Jan 31;103(6):1936–1940. doi: 10.1073/pnas.0510710103

Fig. 5.

Fig. 5.

PLD1 corrects the impaired neurite outgrowth/branching capacity in FAD mutant neurons. (a) Embryonic day 17 cortical neurons (PS1wt or FAD-linked PS1 mutant M146V) were transfected with PLD1wt, PLD1 K898R, or control GFP and then plated onto myelin slides for neurite outgrowth assays, followed by immunolabeling for GAP43. (b) Quantification of the longest neurites from 200 to 300 positively double-immunolabeled neurons (GAP43 and HA-PLD1 or GAP43 and GFP control) and quantification of the number of total processes (more than two cell bodies in length) projecting from each positive neuron. ∗, P < 0.01; ∗∗, P < 0.001, Student’s t test. (Scale bar, 10 μm.)