Fig. 2.

Effect of catalytically inactive WNK3-D294A on K-Cl cotransport under isotonic conditions. X. laevis oocytes were injected with water or 0.2 μg/μl each of the KCCs cRNAs alone or together with WNK3-DA cRNA. ⁸⁶Rb⁺ uptake was assessed 4 days later in isotonic conditions (220 mOsm/kg) in the presence (open bars) or absence (filled bars) of Cl⁻ in the uptake medium. A representative experiment from a single frog, with the mean ± SEM of 15 oocytes for each group, is shown. Similar results have been observed in at least five different experiments, each experiment using oocytes from a different frog. ∗, significantly different from the uptake observed in the corresponding control (absence of WNK3-DA, P < 0.01).