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. 2006 Feb 7;103(7):2380–2385. doi: 10.1073/pnas.0506668103

Fig. 3.

Fig. 3.

TGF-β1 up-regulates expression of α5 integrin and Fn by epithelial cells. (A) HEp-2 cells were pretreated with TGF-β1 and lysed in RIPA buffer (Supporting Text, which is published as supporting information on the PNAS web site) at indicated time. Equal amounts of protein were separated by SDS∕PAGE and blotted with anti-α5, anti-Fn, anti-phospho-smad2 (P-Smad 2), anti-β1, or anti-actin. (B) HEp-2 cells were not treated (Ctrl) or treated with TGF-β1 for 24 h after pretreatment with the inhibitor of TGF-β1 type I receptor SB 431542 (10 μM), (SB 431542∕TGF-β1) or DMS (10 μM) (inhibitor solvent control), (DMSO∕TGF-β1) or medium alone (TGF-β1) for 60 min, then infected with streptococci. Data are presented as described in Fig. 1. ∗, P < 0.02; ∗∗, P < 0.04. (C) HEp-2 cells were not treated (Ctrl) or pretreated with TGF-β1 and then incubated with anti-α5 integrin and anti-Fn and indicated corresponding control antibodies for 30 min before infection with bacteria. ∗, P < 0.02; ∗∗, P < 0.04 compared with Ctrl.