Fig. 4.

Asynchronous and overinitiation in interacting mutant strains. Two distinct assays were used: (A) Flow-cytometry analysis of DNA contents in yabA mutant cells. Comparison of histograms of DNA content in the CRK6000 background for wild-type yfp–yabA⁺, mutant ΔyabA yfp–yabA–Aim (N85D), and ΔyabA yfp–yabA–Nim (L110P) strains. (B) Visualization of origin regions. Spo0J-GFP foci were used to monitor in the 168 background the localization pattern of the origin region in the yabA+, ΔyabA, yabA-Aim, and yabA-Nim strains. Overlay of Spo0J-GFP foci (green) and FM5–95 membrane staining (red).