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Complementation of YabA-Nim initiation defect by YabA-Aim. Spo0J-GFP foci were observed in exponentially growing ΔyabA and yabA-Nim cells harboring a plasmid expressing the wild-type YabA or the YabA-Aim proteins from an IPTG-inducible Pspac promoter (as indicated at left). Optimal complementation was achieved with 40 μM IPTG, corresponding to the level of YabA protein visualized in Fig. 8D. An analysis of the distribution Spo0J foci in the cell population is presented in Table 4.
