Fig. 3.
AEBP1 down-regulates major macrophage cholesterol homeostasis mediators. Protein extracts from AEBP1TG and AEBP1NT macrophages (A), as well as AEBP1+/+ and AEBP1−/− macrophages (C), were subjected to immunoblotting. Densitometric analysis based on actin expression in AEBP1+/+ and AEBP1−/− macrophages (B), as well as in AEBP1+/+ and AEBP1−/− macrophages (D), is shown. (E–H) Semiquantitative RT-PCR was performed on RNA samples obtained from AEBP1TG and AEBP1NT macrophages (E), as well as AEBP1+/+ and AEBP1−/− macrophages (G). Densitometric analysis based on β-actin level in AEBP1TG and AEBP1NT macrophages (F), as well as AEBP1+/+ and AEBP1−/− macrophages (H), is shown. Statistical significance was determined relative to protein expression level or mRNA level in AEBP1NT or AEBP1+/+ macrophages.