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. 2006 Feb 6;103(7):2346–2351. doi: 10.1073/pnas.0508139103

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© 2006 by The National Academy of Sciences of the USA

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Fig. 4. — Enhanced macrophage inflammatory responsiveness by AEBP1. (A–D) ELISA was performed to evaluate the secretion of IL-6 (A and C) and TNF-α (B and D) by macrophages. (E–H) Semiquantitative RT-PCR was performed to assess MCP-1 and iNOS expression in AEBP1^TG and AEBP1^NT (E) and AEBP1^+/+ and AEBP1^−/− (G) macrophages. Histograms illustrating MCP-1 and iNOS mRNA levels in AEBP1^TG and AEBP1^NT (F) and AEBP1^+/+ and AEBP1^−/− (H) macrophages are shown. Statistical significance was determined relative to IL-6 and TNF-α secretion and MCP-1 and iNOS mRNA levels in AEBP1^NT (A, B, and F) and AEBP1^+/+ (C, D, and H) macrophages.