Fig. 3.

Long-term hematopoietic reconstitution activities of G⁺L⁻S⁺K⁺ and equivalent GFP⁻ cells. (A) The contribution of donor (Ly5.1⁺ EIS-KI mice)-derived myeloid (Mac⁺ and Gr1⁺, Left) and lymphoid (Thy1.2⁺ and B220⁺, Right) cells in lethally irradiated Ly5.2⁺ recipient mice was measured for 8 months after transplantation. Total Lin⁻ cells (2 × 10⁵, dotted lines/triangles), 4 × 10³ Lin⁻GFP⁺ (black circles), or 2 × 10⁵ Lin⁻GFP⁻ (gray boxes) cells were examined. (B) The frequency of long-term (5 months) reconstituting HSC in G⁺L⁻S⁺K⁺ (black circles) and comparable GFP⁻ (gray boxes) fractions was investigated by limiting dilution assay. The gray lines show the 37%-negative points based on a Poisson distribution. (C) Bone marrow cells recovered from primary recipients were divided into GFP⁺ and GFP⁻, and 2 × 10⁴ cells from each population were secondary transplanted. The chimerism of transplanted cells in the peripheral myeloid (Left) and lymphoid (Right) compartments after transplantation is shown.