Table 2.
Comparison of GATA-2 expression with GFP expression
| Tissues | GATA-2 (RT-PCR)* |
GFP (FM/IHC)† |
|||
|---|---|---|---|---|---|
| eIV/eV | eIS/eII | eIG/eII | EII-KI | EIS-KI | |
| Embryonic | |||||
| E7.5 | + | − | + | Trophoectoderm, extraembryonic mesoderm | — |
| E9.5 | + | ± | + | mb, ec, ys, aorta, ur, pl, ht | mb |
| E11.5 | + | + | + | liver, brain, sc, ec, ys, aorta, ur, pl, ht, ear, nose | Liver, brain, sc |
| E13.5 | + | + | + | liver, brain, sc, ec, kidney, urogenital, pl, ht, ear, nose | Liver, brain, sc |
| Adult | |||||
| Brain | + | + | + | n, ec | n |
| Thymus | − | − | − | ec | — |
| Heart | + | − | + | ec | — |
| Lung | + | + | + | ec, nd | nd |
| Liver | − | − | − | ec | — |
| Spleen | + | + | + | h, ec | h |
| Kidney | + | + | + | ec, tubular cell, nd | nd |
| Uterus | + | + | + | Endometrium, ec | Endometrium |
| Ovary | − | − | − | ec | — |
| Testis | + | − | + | ec, nd | — |
*RT-PCR was performed using exon IS, IG, II, IV, and V Gata2 gene-specific primers (eIS, eIG, eII, eIV, and eV, respectively).
†GFP expression in EIS-KI and EII-KI mice was detected by fluorescent microscope (FM) or immunohistochemistry (IHC) using anti-GFP antibody. mb, midbrain; ec, vascular endothelial cell; ys, yolk sac; ur, urogenital ridge; pl, placenta; ht, heart; sc, spinal cord; n, neural cell; h, hematopoietic cell; nd, not determinant cell.