Fig. 3.

N-deglycosylated glycophorin C did not inhibit binding of BAEBL (VSTK) to human erythrocytes. (A) Untreated (GPC) and N-glycanase-treated (N-D GPC) normal glycophorin C and Gerbich-negative glycophorin C were analyzed by Western blotting by using anti-glycophorin C antibody. The positions of molecular mass (kDa) marker proteins are indicated. (B) Immunoprecipitation of BAEBL (VSTK) eluted from human erythrocytes preincubated with either glycophorin C (GPC) or N-deglycosylated glycophorin C (N-Deglycos. GPC). The molecular weight standards are shown on the left. (C) The percent inhibition of binding of VSTK BAEBL to erythrocytes by glycophorin C and N-deglycosylated glycophorin C. Data are shown as the mean of two independent experiments, and the error bar is the standard deviation.