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. 2006 Feb 6;103(7):2310–2315. doi: 10.1073/pnas.0510727103

Fig. 1.

Fig. 1.

Replication of H77-S and JFH-1 RNAs in transfected Huh-7.5 cells. (A) Organization of the H77-S genomic RNA, showing location of the five adaptive mutations and the H77-S∕ΔE1p7 mutant in which sequence encoding the structural proteins was deleted. (B) Semiquantitative RT-PCR assays for HCV RNA in lysates (Left) and supernatant fluids (Right) of transfected Huh-7.5 cells. (C) Immunoblot detection of the HCV core and NS3 proteins in transfected Huh-7.5 cells. (D) Core antigen detected by indirect immunofluorescence 96 h after transfection of Huh-7.5 cells with the indicated RNA.