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. 2006 Feb 6;103(7):2310–2315. doi: 10.1073/pnas.0510727103

Fig. 3.

Fig. 3.

Equilibrium ultracentrifugation of H77-S and JFH-1 particles in isopycnic iodixanol gradients. (A) Semiquantitative RT-PCR detection of HCV RNA in fractions of gradients loaded with concentrated, filtered (0.2 μm) supernatant fluids from cells transfected with the indicated RNAs. (B) Results of infectivity assays (bars) and quantitative TaqMan RT-PCR assays in fractions from gradients loaded with concentrated supernatant fluids from cells transfected with H77-S (solid line with open circles) or H77-S∕ΔE1p7 (dashed line with filled circles) RNA. H77-S∕ΔE1p7 supernatant fluids contained no infectious virus. (C) Results of similar assays using fractions from gradients loaded with concentrated JFH-1 supernatant fluids. (D and E) HCV core antigen detected by indirect immunofluorescence in cells inoculated with fraction 5 of gradients loaded with H77-S (D) or JFH-1 (E) material (lower magnification).