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. 2006 Feb 6;103(7):2274–2279. doi: 10.1073/pnas.0510965103

Fig. 4.

Fig. 4.

MKP-1−/− macrophages show altered levels of IL-10 and TNF-α in response to LPS. (A) BMDM from MKP-1+/+ (open bars) and MKP-1−/− (filled bars) mice were stimulated with 10 ng/ml LPS for the indicated time points, and culture supernatants were harvested for ELISA analysis of IL-10 and TNF-α levels. (B) RNA was isolated and subjected to quantitative RT-PCR analysis of IL-10 and TNF-α levels. (C) BMDM from MKP-1+/+ and MKP-1−/− mice were incubated with (+) or without (−) an IL-10 neutralizing Ab (20 μg/ml) for 1 h, and activated with 10 ng/ml LPS for the indicated time points. Expression of TNF-α mRNA was measured by quantitative RT-PCR analysis. Shown is a representative result from three independent experiments. (D) BMDM from MKP-1+/+ (open bars) and MKP-1−/− (filled bars) mice were stimulated with 10 ng/ml LPS, and expression of Bcl-3 was examined by quantitative RT-PCR analysis.