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. 2006 Feb 14;103(8):2809–2814. doi: 10.1073/pnas.0506854103

Fig. 2.

Fig. 2.

Increased apoptosis and caspase activity in post-IR livers of bi-1 knockout mice. (A) TUNEL Assay. Apoptotic mouse hepatocytes were visualized by TUNEL assay in tissue sections from livers of bi-1−/− (KO) and bi-1+/+ (WT) mice (mean ± SD; n = 5–8). (B–D) Caspase activities. Liver homogenates from ischemic (I) and nonischemic (NI) lobes were prepared from bi-1−/− (KO) and bi-1+/+ (WT) mice after IR injury, normalized for total protein content, and caspase protease activities were measured by using fluorigenic tetrapeptide substrates: Ac-DEVD-AFC (B); Ac-LEHD-AFC (C) and Ac-IETD-AFC (D). Data represent Vmax (mean ± SD; n = 5–8). Statistical significance (∗) was determined by the Mann–Whitney U test (P < 0.05, KO versus WT).