Table 4.
In vivo nude mouse tumorigenicity study showing failure of all elderly donor hTERT cells to form tumors in immunodeficient rodents
| Cell type | PD | No. of mice injected | No. of tumors formed |
|---|---|---|---|
| HEK-293 | — | 12 | 12/12 |
| 57yo hTERT | 14 | 2 | 0/2 |
| 65yo hTERT | 4 | 2 | 0/2 |
| 81yo hTERT | 10 | 2 | 0/2 |
| 85yo hTERT | 14 | 2 | 0/2 |
| High PD 65yo hTERT | 57 | 2 | 0/2 |
| High PD 85yo hTERT | 95 | 2 | 0/2 |
hTERT SMC continued to show a failure to form tumors even when injected at high PD far beyond the point of control cell senescence (up to PD 95 in 85yo hTERT SMC). HEK-293 cells, an established tumor cell line, produced tumors in all injected mice and served as a positive control for tumor-forming ability in each mouse.