Fig. 3.

Allogeneic-DC expanded Tregs suppress the MLR in an antigen-specific manner. CD62L⁺ CD25⁺ CD4⁺ T cells from Thy1.1⁺ B6 mice were freshly isolated or expanded with BALB/c or SJL-DCs. These were mixed with 10⁵ CFSE-labeled, Thy 1.2⁺, B6, and CD25⁻ CD4⁺ T cells in various ratios and stimulated with 10⁵ irradiated spleen cells from BALB/c (A) or SJL mice (B). Five days later, CFSE dilution was analyzed by FACS. The displayed cells were gated on live Thy1.2⁺ cells, and the percent of divided CFSE-low live Thy1.2⁺ cells is shown inside the plot. (C) As in B, but numbers of live Thy1.2⁺ responder cells per culture. One of two similar experiments.