Fig. 1.
Expression of recombinant GST-vhs fusion protein and specificity of anti-vhs antiserum. (A) Coomassie blue-stained gel of GST-vhs fusion protein bound to GS and eluted with buffer containing 75 mM Hepes (pH 7.4), 150 mM NaCl, 5 mM DTT, and 0.08% SDS before (lane 2) and after (lane 3) dialysis against buffer lacking SDS. The molecular weight marker is also shown (lane 1). (B) HeLa and Hep2 cells were either mock infected (lane 1) or infected with 10 PFU/cell of HSV-1(F) (lane 2), ΔUL41 mutant virus (lane 3) or ΔUL41R repaired virus (lane 4). Cells were harvested 18 h after infection and subjected to electrophoretic separation in denaturing gels and immunoblotting with the anti-vhs rabbit antiserum.