Fig. 2.
Activation of tissue-selective promoters by TAF4b. (A) Luciferase assays demonstrate the activation of GC-specific promoters in Flag-TAF4b overexpressing SIGCs (gray bars) compared with flag control cells (white bars). Fold activation was obtained by comparison of relative luciferase activity measured from f-TAF4b stable cells versus control flag cells. Error bars represent SDs from at least three independent experiments. (B) The autoradiography of RPAs using RNA isolated from f-TAF4b and flag stable SIGCs and detection of the endogenous Inhba mRNA or an internal control Gapd mRNA. Control, yeast RNA. (C) Luciferase assays demonstrate a specific activation of GC-selective promoters in NIH/3T3 cells that overexpress Flag-TAF4b (gray bars) compared with flag control cells (white bars). Fold induction was measured as described in A.