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. 2006 Feb 10;103(8):2594–2599. doi: 10.1073/pnas.0510764103

Fig. 4.

Fig. 4.

Phenotype of SIGCs and ovaries that lack TAF4b expression. (A) SIGCs were treated with TAF4b and control siRNAs for the indicated time points, followed by immunoblot analysis using anti-TAF4, anti-TAF4b, anti-c-Jun, and anti-β-tubulin antibodies as a loading control. (B) Treatment of SIGCs with TAF4b (+) in comparison with control (–) siRNAs reduces c-jun expression. Gene expression was determined by real-time RT-PCR analysis of RNAs from TAF4b siRNA-treated cells after standardization to control siRNAs and using Gapd as an internal control. Error bars represent SDs from three independent experiments. (C) Live-cell images of siRNA-treated SIGCs at ×10 magnification. An occasional fibroblastic cell can be identified in control siRNA-treated cells (arrows). (D) Hematoxylin-and-eosin-stained ovary sections are shown. Disorganized GCs surrounding an oocyte (Oo) are evident in Taf4b−/− follicles compared with wild-type Taf4b+/+ follicles, which are demarcated with a dotted line.