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. 2006 Feb 10;103(8):2677–2682. doi: 10.1073/pnas.0511084103

Fig. 2.

Fig. 2.

Z-DNA-induced mutations in bacterial cells and mammalian COS-7 cells. (A) Z-DNA-induced lacZ′ mutation frequencies in DH5α cells. (B) Z-DNA-induced lacZ′ mutation frequencies in mammalian COS-7 cells. (C) Z-DNA-induced supF mutation frequencies in DH5α cells. (D) Z-DNA-induced supF mutation frequencies in mammalian COS-7 cells. A total of >100,000 colonies were counted in each group. Error bars show the SEM of three or four independent experiments. (E) pUCG(14) mutants derived from DH5α cells (Left) and COS-7 cells (Right) were digested with EagI and BssSI, resulting in seven fragments. The 877-bp fragment containing the Z-DNA-forming sequence (Z) and the 2,302-bp fragment between the Z-DNA locus and SV40 Ori (SV) are shown schematically (Upper) and marked with arrows in the gel. ∗ indicates large-scale deletions (≥50 bp); C refers to control sample.