Phylogenetic relationships of the HA (A) and NP (B) genes of representative influenza A viruses isolated in Asia. Trees were generated by the neighbor-joining method in the paup* program (35) (maximum-likelihood and Bayesian analysis revealed the same relationships). Numbers above and below branches indicate neighbor-joining bootstrap values and Bayesian posterior probabilities. Not all supports are shown due to space constraints. Analysis was based on nucleotides 1–1696 of the HA gene and 1–990 of the NP gene. The HA tree was rooted to Gs/GD/1/96 and the NP tree to Dk/HK/Y280/97. (Scale bar: 0.01 substitutions per site.) Purple, viruses from Indonesia; red, viruses from Vietnam, Thailand, and Malaysia; green (genotype V) and blue (genotype Z), viruses isolated from migratory birds; brown, viruses isolated from poultry in Hunan and Yunnan in late 2004 and early 2005. GD, Guangdong; HN, Hunan; IDN, Indonesia; MB, migratory bird; YN, Yunnan; VNM2, recent Vietnam introduction; VTM, Vietnam/Thailand/Malaysia. ∗, Because 90% of the poultry consumed in Hong Kong was imported from Guangdong, the viruses isolated from domestic and migratory birds in Hong Kong were designated as belonging to the GD sublineage.