Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Feb 21;103(9):3422–3427. doi: 10.1073/pnas.0507710103

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2006 by The National Academy of Sciences of the USA

PMC Copyright notice

Fig. 5. — Activated Gαq subunits coassociate with TASK channels. Cell lysates and HA-immunoprecipitates (HA-IP) were obtained from HEK293 cells cotransfected with the indicated HA-tagged TASK channel subunits (T1, TASK-1; T3, TASK-3) and either WT (q) or constitutively active Gαq (q*). After SDS/PAGE, immunoblotting for Gαq revealed only active Gαq* in the HA-IP (Left, arrow), but both Gαq and Gαq* in the cell lysate (Center); both HA-tagged TASK channels were obtained in the HA-IP (Right, arrowhead). TASK-3 channels expressed at higher levels than TASK-1 channels, as reflected in the HA blot. Blots are representative of at least four replicate experiments.