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. 2006 Feb 16;103(9):3262–3267. doi: 10.1073/pnas.0507783103

Fig. 3.

Fig. 3.

RNAs accumulate in the absence of RRP6 or TRF4. Northern blots detect transcripts from genomic loci. (A) rrp6Δ. Five micrograms of total RNA from wild-type (lane 1) and rrp6Δ (lane 2) strains, 300 ng of poly(A)+ RNA from rrp6Δ strain (lane 3), and 5 μg of poly(A)− RNA from rrp6Δ strain (lane 4) were loaded onto a 1% agarose-formaldehyde gel and probed with the indicated regions. (B) trf4Δ. Five micrograms of total RNA from wild-type (lane 1) and trf4Δ (lane 2) strains, 300 ng of poly(A)+ RNA from trf4Δ strain (lane 3), and 5 μg of poly(A)− RNA from trf4Δ strain (lane 4) was used. Annotations for the region are indicated on the left, and the probed region is indicated by the black bar. Dubious ORFs are shaded in gray. The name of the candidate region and size of the heterogeneous RNAs are indicated on the right.