RNAs accumulate in the absence of RRP6 or TRF4. Northern blots
detect transcripts from genomic loci. (A)
rrp6Δ. Five micrograms of total RNA from
wild-type (lane 1) and rrp6Δ (lane 2) strains,
300 ng of poly(A)+ RNA from rrp6Δ
strain (lane 3), and 5 μg of poly(A)− RNA from
rrp6Δ strain (lane 4) were loaded onto a
1% agarose-formaldehyde gel and probed with the indicated
regions. (B) trf4Δ. Five
micrograms of total RNA from wild-type (lane 1) and
trf4Δ (lane 2) strains, 300 ng of
poly(A)+ RNA from trf4Δ strain (lane
3), and 5 μg of poly(A)− RNA from
trf4Δ strain (lane 4) was used. Annotations
for the region are indicated on the left, and the probed region is
indicated by the black bar. Dubious ORFs are shaded in gray. The name
of the candidate region and size of the heterogeneous RNAs are
indicated on the right.