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. 2006 Feb 16;103(9):3262–3267. doi: 10.1073/pnas.0507783103

Fig. 4.

Fig. 4.

SRG1 is a promoter associated RNA whose decay requires Rrp6p. (A) Northern blot to detect the levels of poly(A)+ and poly(A)− SRG1 RNA (Upper): 3 μg of total RNA from wild type, trf4Δ, and rrp6Δ (lanes 1, 4, and 7), 100 ng of poly(A)+ RNA from wild type, trf4Δ, and rrp6Δ (lanes 2, 5, and 8), and 3 μg of poly(A)− RNA from wild type, trf4Δ, and rrp6Δ (lanes 3, 6, and 9). The membrane was probed with an SRG1-specific oligo. (Lower) A duplicate membrane probed with an oligo complementary to snR128. (B) Northern blot analysis of SER3 in different yeast mutants. Three micrograms of total RNA from wild-type (lane 1), trf4Δ (lane 2), rrp6Δ (lane 3), heat-shifted spt5-194 (lane 4), heat-shifted spt5-194, rrp6Δ (lane 5), and snf2Δ (lane 6) yeast were fractioned on a 1% agarose formaldehyde gel. The membrane was probed with an SRG1+SER3 oligo complementary to a region contained in both SRG1 RNA and SER3 mRNA.