Table 1.
DLS data for assemblies of fragments 1 and 2
| Entry | Peptide | Solvent | Temperature, °C | Rh, nm (SDP weight analysis) | Length, nm (32–34) | Length, nm (35, 36) | Estimated monomer units* | Rh, nm (SDP intensity analysis) |
|---|---|---|---|---|---|---|---|---|
| Low temperature | ||||||||
| 1 | 1 | 50 mM HOAc(aq) | 10 | 3.1 ± 0.2 | 14 ± 2 | 18 ± 2 | 2 | 3.3, 72 |
| 2 | 2 | 50 mM HOAc(aq) | 10 | 4.0 ± 0.1 | 22 ± 1 | 26 ± 1 | 3 | 5.2, 65 |
| 3 | 1 | 67:33 MeOH/HOAc(aq) | 10 | 5.4 ± 0.5 | 35 ± 5 | 40 ± 5 | 4–5 | 7.5, 43, 200, 650 |
| 4 | 2 | 67:33 MeOH/HOAc(aq) | 10 | 7.6 ± 1.1 | 58 ± 12 | 63 ± 12 | 7–8 | 14, 600 |
| High temperature | ||||||||
| 5 | 1 | 50 mM HOAc(aq) | 60 | 1.9 ± 0.2 | 5 ± 1 | 8 ± 2 | 1 | 2.3, 78 |
| 6 | 2 | 50 mM HOAc(aq) | 60 | 2.1 ± 0.4 | 7 ± 2 | 10 ± 3 | 1 | 2.4, 92 |
| 7 | 1 | 67:33 MeOH/HOAc(aq) | 55 | 1.6 ± 0.6 | 4 ± 3 | 6 ± 4 | 1 | 6.5, 63, 230 |
| 8 | 2 | 67:33 MeOH/HOAc(aq) | 55 | 3.1 ± 0.5 | 14 ± 4 | 18 ± 4 | 2 | 3.7, 900 |
*Estimates are based on the 8.6-nm length of a (ProProGly)10 segment in a crystalline triple helix (41).