Fig. 5.

ABCA1-induction is decreased in LXRα/β^−/− phagocytes: joint role of TNFα and LXR in induction of ABCA1 in phagocytes. Thioglycollate-elicited peritoneal macrophages were isolated from LXRα/β^−/− mice and their control WT littermates and cultured as described in Materials and Methods. Macrophage monolayers (WT or LXRα/β^−/−) were incubated with WT FC-AMs in the presence or absence of anti-TNFα Ab (10 μg/ml), and RNA extracts were performed, as described in Fig. 4. ABCA1 (A), ABCG1 (B), and ABCA7 (C) mRNA were analyzed by real-time quantitative PCR, and data are shown normalized for housekeeping genes. ∗, P < 0.05; ∗∗, P = 0.056. The experiment was conducted in two different cell preparations; the graphs show the average of triplicates of wells of one representative experiment.