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. 2006 Feb 21;103(9):3100–3105. doi: 10.1073/pnas.0510842103

Fig. 4.

Fig. 4.

Differential mode of p160 factors for the DHT- versus SARM-dependent activation. (A) Injection of anti-SRC-1 IgG blocked both the DHT-dependent and bicalutamide/IL-1β-dependent activation of a reporter containing ARE. (B and C) After microinjection of anti-SRC-1 IgG, each LXXLL mutant of SRC-1 plasmid rescued the activation in the presence of DHT (B) or bicalutamide and Il-1β (C). Effects of mutating each LXXLL motif in SRC1 on DHT-dependent and bicalutamide/IL-1β-dependent activation are shown. (D) LNCaP cells stably infected with the AR virus or the control vector were generated and were starved for 5 days and then challenged with bicalutamide (10 μM), R1881 (100 pM), or DHT (10 nM). After 1 h of treatment, ChIP assay was performed with various IgGs. (E) Schematic representation of the action of coactivator use in SARMs-dependent gene activation.