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. 2006 Feb 16;103(9):3274–3279. doi: 10.1073/pnas.0511113103

Fig. 5.

Fig. 5.

ERK activation is required for the TLR ligand-mediated induction of IL-10 in BMDMs. (A and B) Wild-type (open bars) and nfkb1−/− (filled bars) BMDMs were treated with LPS or CpG (A) or LPS in the presence or absence of PD98059 (PD) or U0126 (UO) (B) for 6 h. (C) BMDMs from both genotypes (identification codes as above) expressing the Raf:ER fusion protein were treated with LPS or CpG in the presence or absence of 4-HT for 6 h. After treatments, culture supernatants were harvested and analyzed for IL-10 by ELISA. These assays were performed in triplicate ± SD for each experiment. (D) Wild-type and nfkb1−/− BMDMs expressing the Raf:ER fusion protein were treated with LPS in the presence or absence of 4-HT for 30 min, lysed, and the presence of phospho-ERK or ERK was determined by Western blotting. Un and ND refer to untreated and undetectable, respectively. This data are representative of three independent experiments.