Table 2.
Fragment ions from the top-down experiment of dSLBP RPD (Mrdet: 13,817.51 Da) localizing and identifying posttranslational modifications
| Fragment ions | Sequence | Δm1, ppm |
|---|---|---|
| y19 | PPTQARDTAKDSNSDSDSD* | 1.5 |
| y19-H3PO4 | [PPTQARDTAKDSNSDSDSD*] | 1.7 |
| y19-2x H3PO4 | [PPTQARDTAKDSNSDSDSD*] | 0.42 |
| b10 | Ac-SYYHHHHHHD | −0.48 |
| b12 | Ac-SYYHHHHHHDYD | −0.30 |
| b13 | Ac-SYYHHHHHHDYDI | −0.96 |
| b15 | Ac-SYYHHHHHHDYDIPT | −0.05 |
| b17 | Ac-SYYHHHHHHDYDIPTTE | −0.09 |
| b19 | Ac-SYYHHHHHHDYDIPTTENL | −0.60 |
| b20 | Ac-SYYHHHHHHDYDIPTTENLY | −0.60 |
| b21 | Ac-SYYHHHHHHDYDIPTTENLYF | −1.1 |
| b22 | Ac-SYYHHHHHHDYDIPTTENLYFQ | −0.63 |
*Four times phosphorylated.