Figure 5.

Single-stranded DNA profiles of S. pombe chromosomes (higher resolution profiles are shown in Fig. S3). Chromosomal coordinates were downloaded from the from the Sanger Center (sequence version AL137130.1) and from the National Center for Biotechnology Information. Chromosome I is shown in two halves as “Ia” and “Ib”. The ratio of ssDNA (S/G1) was plotted as a function of chromosomal coordinates for wild type (purple, Y1 axis) and Δcds1 (orange, Y2 axis) cells. The approximate position of each centromere is marked by a grey circle. Purple circles, locations of significant ssDNA peaks identified from wild type cells. Orange circles, locations of significant ssDNA peaks identified from Δcds1 cells. Standard deviation for baseline variation was determined similarly as described for S. cerevisiae (see Supplementary Information, Identification of significant ssDNA peaks). A given local maximum is considered a significant peak if the peak height differential is above 2 standard deviations from either of its flanking local minima. The positions of known origins that have been identified as significant ssDNA peaks are marked by vertical grey lines¹⁹ and the origin names are listed above the graphs. The two known origins that were not detected by a significant ssDNA peak, pcr1 and pARS727, are each marked by a box.