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. Author manuscript; available in PMC: 2006 Mar 27.
Published in final edited form as: J Immunol. 2005 Aug 15;175(4):2449–2458. doi: 10.4049/jimmunol.175.4.2449

FIGURE 3.

FIGURE 3

Phosphorylation kinetics of various signaling proteins in human PBLs. A, Human PBLs were treated with anti-CD3 alone, anti-CD3 and anti-CD4, or anti-CD3 and anti-CD28. The tyrosine phosphorylation of specific sites on LAT was then determined by immunoblotting as described in Materials and Methods. B, Human PBLs were treated with or without anti-CD3 and anti-CD4 for various times and the tyrosine phosphorylation of specific sites on LAT, along with the expression level of GAPDH, was determined by immunoblotting as described in Materials and Methods. C, The percent activation at 120 s for specific LAT tyrosines, as examined in B, was determined as described in Materials and Methods. The mean ± SEM of three separate experiments for each time point was plotted and fit using Origin.