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. Author manuscript; available in PMC: 2006 Mar 27.
Published in final edited form as: J Immunol. 2005 Aug 15;175(4):2449–2458. doi: 10.4049/jimmunol.175.4.2449

FIGURE 5.

FIGURE 5

Phosphorylation kinetics of various signaling proteins in JCaM 2.5 Jurkat cells. JCaM2.5 Jurkat cells were treated with or without anti-CD3 for various times and the cellular proteins were separated by PAGE. A, The tyrosine phosphorylation of specific sites on ZAP-70 and c-Cbl, along with the expression level of GAPDH, was determined by immunoblotting. For comparison, the phosphorylation of these proteins and the expression level of GAPDH in E6.1 Jurkat cells treated with anti-CD3 for 120 s are shown. B, The tyrosine phosphorylation of specific sites on PLC-γ1 and SLP-76, along with the expression level of GAPDH, was determined by immunoblotting. For comparison, the phosphorylation of these proteins and the expression level of GAPDH in E6.1 Jurkat cells treated with anti-CD3 for 120 s are shown. C, The percent activation of E6.1 Jurkat cells at 120 s for PLC-γ1 and SLP-76 in E6.1 and JCaM 2.5 Jurkat cells was determined. The mean ± SEM of three separate experiments for each time point was plotted and fit using Origin.