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The Dependence of fluorescence maximum emission wavelength on the excitation wavelength. While Nile Red maleimide in a 0.1 molar ratio of methanol in water (triangles) did not change its emission wavelength, both Cys261-NR (circles) and unfolded MDH loaded Cys261-NR (open diamonds) showed red shifts in maximum emission wavelength as excitation wavelength increased.
