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Sequence dependence of the time-dependent fluorescence intensity changes of Cys261-NR with ADP/AlFx. The fluorescence intensity at maximum was monitored while adding GroES and ADP/AlFx, with unfolded MDH addition at the end. (A). The sequence ES-ADP/AlFx-MDH. (B). The sequence ADP/AlFx-ES-MDH. (C) Same as (A), except long incubation (45 minutes) with ADP/AlFx, (D) Same as (B), except 1:1 molar ratio of GroEL:GroES.
