Table 3.
Comparison of DNA microarray data with expression data from quantitative real time PCR
| Array data | Real time PCR repeat 1 | Real time PCR repeat 2 | ||||||||
| GenBank ID | Gene | A | B | C | A | B | C | A | B | C |
| AJ780552 | Cysteine protease | 6.3 | 26.8 | 17.8 | 68.4 | 100.4 | 365.8 | 89.9 | 340.1 | 130.2 |
| AJ780698 | Cyclic nucleotide and calmodulin-regulated ion channel | 4.7 | 8.9 | 7.7 | 21 | 13.6 | 18.4 | 15.3 | 35.3 | 21.9 |
| AJ780435 | Aldehyde dehydrogenase | 4.0 | 4.3 | 4.8 | 5.2 | 4 | 3.1 | 6.8 | 11.2 | 15.5 |
| AJ769631 | ATP-dependent Clp protease ATP-binding subunit clpA | 1.6 | 2.0 | 2.4 | 2.1 | 1.5 | 1.9 | 2 | 1.2 | 0.8 |
| AJ778655 | Alcohol dehydrogenase | 0.5 | 0.7 | 1.1 | 0.6 | 0.6 | 1.1 | 0.8 | 1.1 | 1 |
| AJ777239 | Stable protein/bspA | 0.4 | 0.2 | 0.5 | 0.3 | 0.1 | 0.03 | 0.3 | 0.1 | 0.05 |
| AJ768555 | Dehydration-responsive protein RD22 | 0.02 | 0.02 | 0.02 | 0.02 | 0.02 | 0.03 | 0.02 | 0.04 | 0.03 |
Two biological repeats were independently reverse transcribed and amplified. The raw threshold cycle (Ct) values were normalized against a glucosidase alpha subunit standard to get normalized ΔCt values, which were used to calculate the fold change in expression between areas A, B, and C compared to area P.