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SP-B analysis. (A) Representative Coomassie blue stained gel, (B) representative western blot analysis, and (C) summary of SP-B ELISA values for control and oxidized BLES, as well as for the isolated proteins from BLES, H∼BLES, and F∼BLES. A total of 2 μg of protein as detected by Lowry were applied to each lane, as described in Experimental Procedures. Comparisons between two groups were made using paired Student t-test (*, p < 0.05; **, p < 0.01).
