TABLE 1.

Effect of BLES oxidation on phospholipid classes

	BLES	H-BLES	F-BLES	PL	H∼PL	F∼PL
LPC	1.37 ± 0.27	6.43 ± 1.11**	5.03 ± 0.76**	0.96 ± 0.12	11.08 ± 0.01**	9.19 ± 0.063**
SM	1.81 ± 0.35	1.89 ± 0.31	1.93 ± 0.40	1.59 ± 0.00	2.45 ± 0.03*	1.75 ± 0.05
PC	79.76 ± 2.82	60.97 ± 2.17*	58.88 ± 2.32**	72.30 ± 1.98	49.50 ± 3.19**	50.00 ± 2.23**
PE	2.43 ± 0.55	3.30 ± 0.80	3.53 ± 0.62	0.49 ± 0.03	2.85 ± 0.09*	6.27 ± 0.21**
LPG	1.29 ± 0.29	3.75 ± 0.16**	4.03 ± 0.77**	1.32 ± 0.03	1.37 ± 0.04	1.41 ± 0.04
PI	1.04 ± 0.29	0.38 ± 0.16	0.35 ± 0.31	2.37 ± 0.19	3.55 ± 0.12	3.37 ± 0.03
PG	14.10 ± 1.10	9.02 ± 0.22**	8.28 ± 0.26**	11.39 ± 0.44	7.96 ± 0.28*	6.27 ± 0.13*
LBPA	1.68 ± 0.16	2.25 ± 0.06*	2.23 ± 0.16*	1.51 ± 0.49	0.67 ± 0.00	2.05 ± 0.10
origin	1.12 ± 0.25	7.36 ± 0.84*	9.60 ± 0.65*	1.56 ± 0.27	8.76 ± 0.24*	8.39 ± 0.48*

Control: H∼BLES and F∼BLES were extracted and the recovered PLs were separated on TLC before and after isolation by LH-60 chromatography. PL phosphorus was measured as indicated in Methods. Recoveries of applied lipid were: C∼BLES, 98.5 ± 1.5; H∼BLES, 87.8 ± 4.7; F∼BLES, 85.9 ± 6.4; C∼PL, 96.5 ± 8.2; H∼PL, 85.9 ± 6.4; F∼PL, 90.1 ± 3.4 (n = 3). Abbreviations: LPC, lysophosphatidylcholine; SM, sphingomyelin; PC, phosphatidylcholine; PE, phosphatidylethanolamine; LPG, lysophosphatidylglycerol; LBPA, lyso-bis-phosphatidic acid.

^*p < 0.05; **p < 0.01.