FIGURE 4.

Deflection measurement of bR-functionalized cantilevers (solid circles) versus blank gold cantilevers (open circles). From the 6574 data points, only 30 are labeled and attributed with an error bar indicating the standard deviation of the averaging (three deflection measurements each). Section I, buffer equilibration for baseline; Section II, incubation time with 200 mM hydroxylamine; Section III, after rinsing with buffer. To obtain normalized deflection values (in nanometers), the deflection was first divided with the peak height of the heat test and then multiplied with the average peak height (see Methods).