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When the Ton box is disturbed, it may be refolded by solutes. EPR spectrum of (a) T7R1 in POPC, (b) T7R1/V10P in POPC, and (c) T7R1/V10P in the crystallization soaking buffer. EPR spectra for N13R1 in (d) POPC and (e) the crystallization soaking buffer. EPR spectra of V10R1 in (f) POPC/OG (1:17) mixed micelles and (g) POPC/OG mixed micelles plus 30% PEG 3350. Spectra are 100 gauss scans.
