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. 2006 Feb 3;90(8):3012–3018. doi: 10.1529/biophysj.105.073114

FIGURE 5.

FIGURE 5

Cultures of dissociated embryonic cortices at 1 week in vitro on soft (A) and hard (B) PA gels and soft (250 Pa) (D) and hard (2.1 kPa) (E) fibrin gels. Neurons were labeled for βIII-tubulin (red) and astrocytes for GFAP (green). All cell nuclei on PA gels were labeled with DAPI (blue). The prevalence of astrocytes is apparent on hard PA gels and to a lesser extent on hard fibrin gels, which are still much softer than the hardest PA gels. The percentage of total cells on soft PA gels (C) and fibrin gels (F) that were neurons (βIII-tubulin positive) was significantly higher than on hard gels (p < 0.01).