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. 2006 Mar 27;103(14):5573–5578. doi: 10.1073/pnas.0601279103

Fig. 4.

Fig. 4.

Glycosylation of Reelin in CSF and plasma. (A) Five nonpathological CSF and plasma samples were incubated with immobilized lectins Con A, LCA, WGA, and RCA. Attempts to assay the Reelin bound to each lectin by resuspending and boiling the resin showed only 60–80% of recovery of the glycoprotein. The unbound Reelin, assayed by Western blotting, in the supernatant fraction was therefore used to compare differences in lectin binding between groups. The data represent the percentages of bound Reelin calculated after subtraction of unbound immunoreactivity for each band. We grouped values into four categories: +++, 75–100% of Reelin binding to lectin; ++, 50–74% binding; +, 25–49% binding; −, 0–24% binding. (B) Lectin binding of 180-kDa Reelin in gender- and age-matched CSF samples for 9 NDC and 11 AD subjects. (C) Scatter plots for the 180-kDa Reelin LCA/Con A ratio (% 180-kDa Reelin unbound to LCA/% 180-kDa Reelin unbound to Con A). The dashed line represents an arbitrary cutoff that maximally discriminated between AD and NDC groups (≈35 arbitrary units). The data represent the means ± SE. ∗, Significantly different (P < 0.05) from the NDC group as assessed by Student’s t test.