Fig. 5.

Reelin carries the HNK-1 carbohydrate. Reelin from frontal cortex (lane 1), CSF (lane 3), and plasma samples (lane 5) was immunoprecipitated by using the G10 antibody (lanes 2, 4, and 6). The experiments were repeated three times. Western blot analyses were performed by using 142 (anti-Reelin; A) and Ab2 (anti-HNK-1; B) antibodies. B shows the immunoreactivity of the 180-kDa cortical frontex (lane 2) for HNK-1 and more weakly for CSF-Reelin (lane 4). In contrast, no HNK-1 band was detected in Reelin immunoprecipitated from plasma (lane 6).