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The Mlp1 protein is excluded from the flare. Strains OCF2189 (SPO7 MLP1-MYC pPUS1-GFP) and JCY545 (spo7Δ MLP1-MYC pPUS1-GFP) were grown to early log phase and processed for immunofluorescence using antibodies against the Myc tag, while maintaining the fluorescence of Pus1-GFP. Mlp1p-Myc, red; DNA, blue; and Pus1-GFP, green. Three typical images of spo7Δ cells are shown.
