Figure 1.

Fluorescence pattern of mitoGFP expressed by the D42 driver. (A) A composite image of a fixed third instar neuromuscular preparation from a larva homozygous for both the D42 Gal4 driver and mitoGFP. GFP fluorescence was strong in the presumptive optic lobes and ventral ganglion (VG), segmental nerves (SN), and neuromuscular junctions (NMJ). In this specimen, the ventral ganglion is lying on its side with optic lobes projecting down. Abdominal segments A2 and A6 are indicated. (B) A high-magnification view of a portion of a single segmental nerve passing through segment A4. Single axonal mitochondria usually were elongated (arrow) but some were small and spherical. Anterior is to the left in this, and all subsequent figures.