Figure 5.

The Golgi is largely functional in statin-treated cells. (A) CHO cells stably expressing GFP-εCOP were treated with 40 μM lovastatin for 1 h or without (control) before confocal microscopy imaging. (B) LPDS-grown CHO cells were transfected with VSVG and incubated at 39.5°C overnight. Cells were then moved to a 19.5°C for 2 h to accumulate VSVG in the Golgi. Statin (40 μM) was added to half of the wells for the last hour. This was followed by 15 min at 32°C before fixing and microscopic observation. (C) LPDS-grown CHO cells were transfected with VSVG and incubated at 39.5°C overnight and statin was added for the hour (b). Cells were then moved to 15°C for 2 h to accumulate VSVG in the ER/Golgi intermediate compartment. Cells were also scored for the appearance of punctate dots (ERGIC) in the cytoplasm (c).