Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Apr;17(4):1971–1984. doi: 10.1091/mbc.E05-10-0956

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, The American Society for Cell Biology

PMC Copyright notice

Figure 4. — Biochemical analysis reveals a severing defect for the aip1-15 mutation. Polymerized actin filaments (2.5 μM) were incubated with or without cofilin and GST-Aip1p (lane 3) or GST-aip1-15p (lane 4) for 10 min. The ratios of actin/cofilin and actin/Aip1p were 1:1 and 20:1, respectively. After high-speed centrifugation (360,000 × g), the extent of filament disassembly was determined based on the amount of actin that remained in the pellet fraction (F-actin) versus the supernatant fraction (G-actin), as detected by SDS-PAGE and SYPRO Ruby staining.