Table 3.

Summary of the phenotypic analyses of aip1 and cof1 mutants

			Two hybrid		Localization
Allele	Mutations	Cell morphology	With actin	With cofilin	Aip1p	Cofilin	Actin organization	Biochemistry
AIP1		WT	+++	+++	Cortical patches	Cortical patches	++++	Normal
aip1Δ		WT	na	na	na	Catches and cables	+++	na
aip1-15	1245A, E246A, D247A, D248A, Q249A, E250A	WT	+	+	Cortical patches	Patches and cables	+++	F-Actin depolym. defects
aip1-34/35	K571A, R572A, K575A/D516A, E521A	1% Elongated buds	+++	+++	Cortical patches	Patches and cables	+++	Normal
aip1-15/34/35	See above	nd	–	–	nd	nd	nd	Normal
aip1-51	H338P, A579T	nd	++	–	nd	nd	nd	nd
aip1-52	T558P	nd	++	–	nd	nd	nd	nd
aip1-53	G271E, D293N	nd	++	–	nd	nd	nd	nd
aip1-55	G449D	WT	++	–	Cortical patches	Patches and cables	+++	Normal
aip1-56	W261Stop	nd	++	–	nd	nd	nd	nd
aip1-59	S492L	1% Elongated buds	++	–	Cytosolic	Patches and cables	+++	Normal
aip1 C-term	G337-E615	nd	±	–	nd	nd	nd	nd
aip1-60	G337-E615, E615K	WT	+	–	Cortical patches	Cortical patches	++++	Normal
aip1-61	G337-E615, F481L	nd	+	–	nd	nd	nd	nd
			With actin	With Aip1p	Aip1p	Cofilin
COF1		WT	+++	+++	Cortical patches	Cortical patches	++++	Normal
cof1-4	S4A	Enlarged	+++	–	Cortical patches	Cortical patches	+	Capping defect
cof1-19	R109A, R110A	Slightly enlarged	++	++	Cytosolic	Cortical patches	++	Severing and capping defects
cof1-13	E59A, D61A	WT	+++	–	Cortical patches	Cortical patches	++++	nd
cof1-22	E134A, R135A, R138A	nd	++	–	Cortical patches	Cortical patches	–	F-Actin depolym.

depolym., depolymerization; na, not applicable; nd, no data; +++, WT growth; ++, moderate growth; +, weak growth; ±, very weak growth; –, no growth.