Table 1.
Strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| LPY5 | MATa | This lab |
| LPY11 | MATasir2Δ::HIS3 | This lab |
| AMR53 | MATatop1-8::LEU2 | R. Sternglanz |
| LPY78 | MATα his4 | This lab |
| LPY2991 | MATahis3Δ200 leu2-3,112 trp1Δ1 ura3-52 esa1Δ::HIS3 + pLP795 | This lab |
| LPY3291 | MATahis3Δ200 leu2-3,112 trp1Δ1 ura3-52 esa1Δ::HIS3 + pLP863 | This lab |
| LPY3498 | MATahis3Δ200 leu2-3,112 trp1Δ1 ura3-52 | This lab |
| LPY3500 | MATahis3Δ200 leu2-3,112 trp1Δ1 ura3-52 esa1Δ::HIS3 esa1-L254P::URA3 | This lab |
| CCF101 | MATα hmr::TRP1 rDNA::ADE2-CAN1 TELVR::URA3 | K. Runge |
| LPY4679 | MATaesa1-414 | This study |
| LPY4680 | MATaesa1-414 | This study |
| LPY4801 | MATa/α +/esa1-414 +/hmr::TRP1 +/rDNA::ADE2-CAN1 +/TELVR::URA3 | This study |
| LPY4804 | MATa/α +/esa1-414 +/hmr::TRP1 +/rDNA::ADE2-CAN1 +/TELVR::URA3 | This study |
| LPY4819 | MATα hmr::TRP1 rDNA::ADE2-CAN1 TELVR::URA3 | This study |
| LPY4821 | MATα esa1-414 hmr::TRP1 rDNA::ADE2-CAN1 TELVR::URA3 | This study |
| LPY4848 | MATα esa1-414 hmr::TRP1 rDNA::ADE2-CAN1 TELVR::URA3 | This study |
| LPY4906 | MATa/α +/esa1-414 +/sir2Δ::HIS3 +/hmr::TRP1 +/rDNA::ADE2-CAN1+/TELVR::URA3 | This study |
| LPY4909 | MATα rDNA::ADE2-CAN1 | This study |
| LPY4911 | MATα esa1-414 rDNA::ADE2-CAN1 | This study |
| LPY4913 | MATα hmr::TRP1 | This study |
| LPY4915 | MATα esa1-414 hmr::TRP1 | This study |
| LPY4917 | MATα TELVR::URA3 | This study |
| LPY4919 | MATα esa1-414 TELVR::URA3 | This study |
| LPY4978 | MATα sir2Δ::HIS3 rDNA::ADE2-CAN1 | This study |
| LPY4980 | MATα sir2Δ::HIS3 hmr::TRP1 | This study |
| LPY5001 | MATaesa1-L254P | This study |
| LPY5017 | MATasir2Δ::TRP1 rDNA::ADE2-CAN1 TELVR::URA3 | This study |
| LPY5055 | MATα esa1-L254P rDNA::ADE2-CAN1 | This study |
| LPY5059 | MATα esa1-L254P hmr::TRP1 | This study |
| LPY5066 | MATα esa1-L254P hmr::TRP1 rDNA::ADE2-CAN1 TELVR::URA3 | This study |
| LPY5099 | MATa/α +/esa1-L254P +/hmr::TRP1 +/rDNA::ADE2-CAN1 +/TELVR::URA3 | This study |
| LPY5136 | MATα esa1-414 rDNA::ADE2-CAN1 + pLP1402 | This study |
| LPY5141 | MATα esa1-414 rDNA::ADE2-CAN1 + pLP796 | This study |
| LPY5143 | MATα esa1-414 rDNA::ADE2-CAN1 + pLP37 | This study |
| LPY5144 | MATα esa1-L254P rDNA::ADE2-CAN1 + pLP1402 | This study |
| LPY5150 | MATα esa1-L254P rDNA::ADE2-CAN1 + pLP796 | This study |
| LPY5151 | MATα esa1-L254P rDNA::ADE2-CAN1 + pLP37 | This study |
| LPY5159 | MATα sir2Δ::HIS3 rDNA::ADE2-CAN1 + pLP1402 | This study |
| LPY5164 | MATα sir2Δ::HIS3 rDNA::ADE2-CAN1 + pLP796 | This study |
| LPY5166 | MATα sir2Δ::HIS3 rDNA::ADE2-CAN1 + pLP37 | This study |
| LPY6181 | MATα TELVR::URA3 + pLP1347 | This study |
| LPY6182 | MATα TELVR::URA3 + pLP1348 | This study |
| LPY6184-7 | MATα TELVR::URA3 + pLP1349 | This study |
| LPY6189 | MATα esa1-414 TELVR::URA3 + pLP1347 | This study |
| LPY6190 | MATα esa1-414 TELVR::URA3 + pLP1348 | This study |
| LPY6192-5 | MATα esa1-414 TELVR::URA3 + pLP1349 | This study |
| LPY6217 | MATα rDNA::ADE2-CAN1 + pLP1347 | This study |
| LPY6219 | MATα rDNA::ADE2-CAN1 + pLP1348 | This study |
| LPY6220-3 | MATα rDNA::ADE2-CAN1 + pLP1349 | This study |
| LPY6225 | MATα esa1-414 rDNA::ADE2-CAN1 + pLP1347 | This study |
| LPY6227 | MATα esa1-414 rDNA::ADE2-CAN1 + pLP1348 | This study |
| LPY6228-31 | MATα esa1-414 rDNA::ADE2-CAN1 + pLP1349 | This study |
| LPY6244 | LPY5 with 3HA-ESA1 | This study |
All strains are in the W303 background with the genotype ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1, and additional pertinent genotype information is noted. Exceptions are the mating tester strain LPY78 and the S288c background strains LPY2991, LPY3291, LPY3498, and LPY3500.