Figure 2.

tTG expression increases cell polarization and promotes a formation of focal adhesions and stress fibers upon adhesion of NIH3T3 fibroblasts on fibronectin in serum-free medium. (A) Cell morphology was visualized by formaldehyde fixation and staining with Coomassie blue. Bar, 50 μm. (B) Cells were double stained for vinculin (green) and F-actin (red). Arrowheads mark focal adhesions that seem more developed in the cells expressing tTG. Bar, 20 μm.