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. 2003 Jan;185(1):221–230. doi: 10.1128/JB.185.1.221-230.2003

FIG. 1.

FIG. 1.

The activity of AcnB, but not AcnA, depends upon iron concentration. (A) Cell extracts containing AcnB (from JRG2789) or AcnA (from JRG3258) were suspended at the indicated protein concentrations in anaerobic 50 mM Tris-HCl and incubated at room temperature. At indicated time points, aliquots were removed and assayed under anaerobic conditions. (B) Cell extracts of JRG2789 (25 mg of protein/ml) containing AcnB were diluted into anaerobic Tris buffer. Where indicated, 1 mM Fe(NH4)2(SO4)2 was included in the buffer. Assay error was <7%; replicate experiments exhibited small variation in inactivation rate.